![]() pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long. Control of cell type in yeast by the mating type locus. Isolation, characterization, and translation of mRNA from yeast. Genetic interactions between MAT alpha and unlinked alpha-specific STE genes. Control of yeast cell type by the mating type locus. Detection of specific sequences among DNA fragments separated by gel electrophoresis. DNA sequencing with chain-terminating inhibitors. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Rigby PW, Dieckmann M, Rhodes C, Berg P.Structure of polyadenylic acid in the ribonucleic acid of Saccharomyces cerevisiae. Phillips SL, Tse C, Serventi I, Hynes N.Structural gene identification utilizing eukaryotic cell-free translational systems. The structure of transposable yeast mating type loci. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. Mating pheromones of Saccharomyces kluyveri: pheromone interactions between Saccharomyces kluyveri and Saccharomyces cerevisiae. Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Structure of a yeast pheromone gene (MF alpha): a putative alpha-factor precursor contains four tandem copies of mature alpha-factor. ![]() ![]() Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. Yeast alpha factor is processed from a larger precursor polypeptide: the essential role of a membrane-bound dipeptidyl aminopeptidase. Julius D, Blair L, Brake A, Sprague G, Thorner J.A versatile primer for DNA sequencing in the M13mp2 cloning system. Plasmid screening at high colony density. Deletion mapping of sequences essential for in vivo transcription of the iso-1-cytochrome c gene. Faye G, Leung DW, Tatchell K, Hall BD, Smith M.Primary structure of the human Met- and Leu-enkephalin precursor and its mRNA. Comb M, Seeburg PH, Adelman J, Eiden L, Herbert E.Transcriptional and translational expression of a chimeric bacterial-yeast plasmid in yeasts. Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate. Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase. Cell-cell recognition in yeast: purification of Hansenula wingei 21-cell sexual agglutination factor and comparison of the factors from three genera. Burke D, Mendonça-Previato L, Ballou CE.Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene. Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Botstein D, Falco SC, Stewart SE, Brennan M, Scherer S, Stinchcomb DT, Struhl K, Davis RW.A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. The primary structure of the Saccharomyces cerevisiae gene for alcohol dehydrogenase. Links to PubMed are also available for Selected References. Get a printable copy (PDF file) of the complete article (2.2M), or click on a page image below to browse page by page. Full textįull text is available as a scanned copy of the original print version. cerevisiae, as well as the sequences detected in the other Saccharomyces species, are indeed expressed and functional genes, and suggest that proper proteolytic processing of prepro-alpha-factor is unaffected by the number of pheromone repeats encoded within this precursor protein. These findings demonstrate conclusively that the putative prepro-alpha-factor DNA cloned from S. The sizes of both the mRNAs and the proteins synthesized from them reflected exactly the differences observed in the lengths of the genes. Analysis of polyadenylated RNA indicated that these genes specified RNA transcripts and that these RNA molecules could be translated in vitro into prepro-alpha-factor polypeptides immunoprecipitable with anti-alpha-factor antibodies. The observed polymorphisms were shown to be due solely to differences in the number of tandemly arranged spacer peptide/pheromone units within the coding sequence of these genes. Analysis by restriction endonuclease digestion and Southern blot hybridization indicated that the physical arrangement of this region is highly conserved in all the Saccharomyces species analyzed, but displays length polymorphisms of limited size (50 to 60 base pairs). The chromosomal region containing a structural gene for the mating pheromone precursor prepro-alpha-factor was examined in a variety of Saccharomyces yeasts by using a cloned putative prepro-alpha-factor gene of Saccharomyces cerevisiae as the probe.
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